British Museum curator Lady Alex Lindo-Parker jets off to the Amazon in search of Victorian explorer Percy Fawcett's lost camp, but after encountering one or two minor mishaps, she is thrown together with maverick American adventurer Hooten. The pair stumble upon El Dorado - the fabled city of gold - but there are rival explorers lurking behind every tree to make sure that getting their hands on any treasure won't be an easy task.

In Rome, Lady Alex helps Hooten search for the Sibylline Books but quickly ends up scaling the Sistine Chapel, battling an alligator and clashing with the mafia.

Lady Alex heads to Egypt, where she teams up with Hooten to find the long-lost tomb of Alexander the Great. But some unscrupulous locals are hot on their heels.