Yoshika launches alone against the out-of-control Warlock, desperately holding out as the other Witches race to join her and put an end to the monstrous hybrid of human and Neuroi technology.

Yoshika's plans to become a doctor are put on hold after a mysterious letter from her father leads to a reunion with Sakamoto. Elsewhere, her former Strike Witch comrades take to the air to battle a new breed of Neuroi!

When Sakamoto's strike unit malfunctions, Yoshika is forced into a solo dogfight against a rapidly-regenerating Neuroi! The bombastic beauties of the 501st Joint Fighter Wing race to provide backup, but will they arrive in time?